Liposomal carrier system incorporating phytosomal forms of silymarin was formulated. Formulations were characterized by particle size, zeta potential, DSC thermogram, XRD data and TEM image. In-vitro release profile showed better release of silymarin from all liposomes compared to free drug with highest release from conventional and DP-liposome at pH 1.2 and from Conventional and PEGylated at pH 7.4. Conventional and PEGylated liposomal formulations showed better protection to Chang liver cells against cytotoxicity of paracetamol. The improved in-vivo function of conventional liposomal formulation was also ascertained by histopathology reports in the tested hepatotoxic models. Conventional liposome showed a significant elevation in antioxidant levels compared to silymarin alone in Wistar rats in all three hepatotoxic models Investigation of the pharmacokinetic results from the oral administration of silymarin and its conventional liposomes showed that conventional liposomes increased Cmax more than five times compared to silymarin alone in normal rats and almost six times in alcoholic liver disease condition in rat. Thus, liposomal formulation of silymarin is a suitable candidate and can be used as liver protectant.
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